Search results for "Bright-field microscopy"
showing 4 items of 4 documents
4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)
2015
AbstractIn the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of…
Light transmission of the human lens and its relation with aging
2011
Purpose To determine the visible light transmission of the human crystalline lens of persons of different ages, using both solar and incandescent illumination. Methods The spectral transmission of lens was measured by using the Perkin-Elmer 800 UV/VIS spectrophotometer. With these data we calculated the percentage of visible light transmission for solar illumination, simulated by the CIE D65 illuminant, and for incandescent illumination (A illuminant). Results The total amount of visible light transmitted by nine crystalline lenses is represented as a function of age: with solar illumination and with incandescent illumination and the comparison between both illuminants.In general, the amoun…
Phase imaging microscopy under the Gabor regime in a minimally modified regular bright-field microscope
2021
Quantitative phase imaging (QPI) is nowadays a powerful tool for visualization and analysis of biological processes. QPI is usually attained from specifically designed optical microscopes retrieving phase information in a quantitative way. In this paper we report on an extremely simple, low cost and compact way to update a standard bright-field microscope with coherent sensing capabilities. It is based on the in-line Gabor holography concept and only needs to replace the illumination broadband source of the regular microscope with a coherent one. The proposed methodology is completed by the recording of a digital in-line Gabor hologram instead of regular imaging conditions and by the numeri…
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy.
2018
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46 NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic…